Jellyfish protein hydrolysates: Multifunctional bioactivities unveiled in the battle against diabetes, inflammation, and bacterial pathogenesis.

This study investigates the multifunctional bioactivities of pepsin-hydrolyzed jellyfish by-products (Rhopilema hispidum and Lobonema smithii), focusing on their anti-α-glucosidase activity, anti-inflammatory effects, anti-bacterial properties, and ability to inhibit biofilm formation of Staphylococcus aureus. Our findings revealed that jellyfish protein hydrolysates, particularly from Rhopilema hispidum, exhibit significant anti-α-glucosidase activity, surpassing the well-known α-glucosidase inhibitor Acarbose. Furthermore, we demonstrated the anti-inflammatory capabilities of these hydrolysates in suppressing lipopolysaccharide (LPS)-induced nitric oxide production in murine macrophage cells. This effect was dose-dependent and non-cytotoxic, highlighting the hydrolysate potential in treating inflammation-related conditions. Regarding anti-bacterial activity, pepsin-hydrolyzed jellyfish selectively exhibited a potent effect against S. aureus, including Methicillin-susceptible and Methicillin-resistant strains. This activity was evident at minimum inhibitory concentrations (MIC) of 25 µg/mL for S. aureus ATCC10832, while a modest effect was observed against other Gram-positive strains. The hydrolysates effectively delayed bacterial growth dose-dependently, suggesting their use as alternative agents against bacterial infections. Most notably, pepsin-hydrolyzed jellyfish showed significant anti-biofilm activity against S. aureus. The umbrella section hydrolysate of Rhopilema hispidum was particularly effective, reducing biofilm formation through downregulating the icaA gene, crucial for biofilm development. Furthermore, the hydrolysates modulated the expression of the agrA gene, a key regulator in the pathogenesis of S. aureus. In conclusion, pepsin-hydrolyzed jellyfish protein hydrolysates exhibit promising multifunctional bioactivities, including anti-diabetic, anti-inflammatory, antibacterial, and anti-biofilm properties. These findings suggest their potential application in pharmaceutical and nutraceutical fields, particularly in managing diabetic risks, inflammation, bacterial infections, and combating the biofilm-associated pathogenicity of S. aureus.

Study of marine microorganism metabolites: new resources for bioactive natural products.

The marine environment has remained a source of novel biological molecules with diversified applications. The ecological and biological diversity, along with a unique physical environment, have provided the evolutionary advantage to the plant, animals and microbial species thriving in the marine ecosystem. In light of the fact that marine microorganisms frequently interact symbiotically or mutualistically with higher species including corals, fish, sponges, and algae, this paper intends to examine the potential of marine microorganisms as a niche for marine bacteria. This review aims to analyze and summarize modern literature data on the biotechnological potential of marine fungi and bacteria as producers of a wide range of practically valuable products (surfactants, glyco-and lipopeptides, exopolysaccharides, enzymes, and metabolites with different biological activities: antimicrobial, antitumor, and cytotoxic). Hence, the study on bioactive secondary metabolites from marine microorganisms is the need of the hour. The scientific novelty of the study lies in the fact that for the first time, the data on new resources for obtaining biologically active natural products - metabolites of marine bacteria and fungi - were generalized. The review investigates the various kinds of natural products derived from marine microorganisms, specifically focusing on marine bacteria and fungi as a valuable source for new natural products. It provides a summary of the data regarding the antibacterial, antimalarial, anticarcinogenic, antibiofilm, and anti-inflammatory effects demonstrated by marine microorganisms. There is currently a great need for scientific and applied research on bioactive secondary metabolites of marine microorganisms from the standpoint of human and animal health.

Marine Bacterial Dextranases: Fundamentals and Applications.

Dextran, a renewable hydrophilic polysaccharide, is nontoxic, highly stable but intrinsically biodegradable. The α-1, 6 glycosidic bonds in dextran are attacked by dextranase (E.C. 3.2.1.11) which is an inducible enzyme. Dextranase finds many applications such as, in sugar industry, in the production of human plasma substitutes, and for the treatment and prevention of dental plaque. Currently, dextranases are obtained from terrestrial fungi which have longer duration for production but not very tolerant to environmental conditions and have safety concerns. Marine bacteria have been proposed as an alternative source of these enzymes and can provide prospects to overcome these issues. Indeed, marine bacterial dextranases are reportedly more effective and suitable for dental caries prevention and treatment. Here, we focused on properties of dextran, properties of dextran-hydrolyzing enzymes, particularly from marine sources and the biochemical features of these enzymes. Lastly the potential use of these marine bacterial dextranase to remove dental plaque has been discussed. The review covers dextranase-producing bacteria isolated from shrimp, fish, algae, sea slit, and sea water, as well as from macro- and micro fungi and other microorganisms. It is common knowledge that dextranase is used in the sugar industry; produced as a result of hydrolysis by dextranase and have prebiotic properties which influence the consistency and texture of food products. In medicine, dextranases are used to make blood substitutes. In addition, dextranase is used to produce low molecular weight dextran and cytotoxic dextran. Furthermore, dextranase is used to enhance antibiotic activity in endocarditis. It has been established that dextranase from marine bacteria is the most preferable for removing plaque, as it has a high enzymatic activity. This study lays the groundwork for the future design and development of different oral care products, based on enzymes derived from marine bacteria.

Cellulolytic and Xylanolytic Enzymes from Yeasts: Properties and Industrial Applications.

Lignocellulose, the main component of plant cell walls, comprises polyaromatic lignin and fermentable materials, cellulose and hemicellulose. It is a plentiful and renewable feedstock for chemicals and energy. It can serve as a raw material for the production of various value-added products, including cellulase and xylanase. Cellulase is essentially required in lignocellulose-based biorefineries and is applied in many commercial processes. Likewise, xylanases are industrially important enzymes applied in papermaking and in the manufacture of prebiotics and pharmaceuticals. Owing to the widespread application of these enzymes, many prokaryotes and eukaryotes have been exploited to produce cellulase and xylanases in good yields, yet yeasts have rarely been explored for their plant-cell-wall-degrading activities. This review is focused on summarizing reports about cellulolytic and xylanolytic yeasts, their properties, and their biotechnological applications.

Alginate Lyases from Marine Bacteria: An Enzyme Ocean for Sustainable Future.

The cell wall of brown algae contains alginate as a major constituent. This anionic polymer is a composite of ß-d-mannuronate (M) and α-l-guluronate (G). Alginate can be degraded into oligosaccharides; both the polymer and its products exhibit antioxidative, antimicrobial, and immunomodulatory activities and, hence, find many commercial applications. Alginate is attacked by various enzymes, collectively termed alginate lyases, that degrade glycosidic bonds through ß-elimination. Considering the abundance of brown algae in marine ecosystems, alginate is an important source of nutrients for marine organisms, and therefore, alginate lyases play a significant role in marine carbon recycling. Various marine microorganisms, particularly those that thrive in association with brown algae, have been reported as producers of alginate lyases. Conceivably, the marine-derived alginate lyases demonstrate salt tolerance, and many are activated in the presence of salts and, therefore, find applications in the food industry. Therefore, this review summarizes the structural and biochemical features of marine bacterial alginate lyases along with their applications. This comprehensive information can aid in the expansion of future prospects of alginate lyases.

Marine Microbial Fibrinolytic Enzymes: An Overview of Source, Production, Biochemical Properties and Thrombolytic Activity.

Cardiovascular diseases (CVDs) have emerged as a major threat to global health resulting in a decrease in life expectancy with respect to humans. Thrombosis is one of the foremost causes of CVDs, and it is characterized by the unwanted formation of fibrin clots. Recently, microbial fibrinolytic enzymes due to their specific features have gained much more attention than conventional thrombolytic agents for the treatment of thrombosis. Marine microorganisms including bacteria and microalgae have the significant ability to produce fibrinolytic enzymes with improved pharmacological properties and lesser side effects and, hence, are considered as prospective candidates for large scale production of these enzymes. There are no studies that have evaluated the fibrinolytic potential of marine fungal-derived enzymes. The current review presents an outline regarding isolation sources, production, features, and thrombolytic potential of fibrinolytic biocatalysts from marine microorganisms identified so far.

Marine microbial L-glutaminase: from pharmaceutical to food industry.

Deamination of L-glutamine to glutamic acid with the concomitant release of ammonia by the activity of L-glutaminase (L-glutamine amidohydrolase EC 3.5.1.2) is a unique reaction that also finds potential applications in different sectors ranging from therapeutics to food industry. Owing to its cost-effectiveness, rapidity, and compatibility with downstream processes, microbial production of L-glutaminase is preferred over the production by other sources. Marine microorganisms including bacteria, yeasts, and moulds have manifested remarkable capacity to produce L-glutaminase and, therefore, are considered as prospective candidates for large-scale production of this enzyme. The main focus of this article is to provide an overview of L-glutaminase producing marine microorganisms, to discuss strategies used for the lab- and large-scale production of these enzyme and to review the application of L-glutaminase from marine sources so that the future prospects can be understood. KEY POINTS: • L-glutaminase has potential applications in different sectors ranging from therapeutics to food industry • Marine microorganisms are considered as prospective candidates for large-scale production of L-glutaminase • Marine microbial L-glutaminase have great potential in therapeutics and in the food industry.

Marine Bacterial Esterases: Emerging Biocatalysts for Industrial Applications.

The marine ecosystem has been known to be a significant source of novel enzymes. Esterase enzymes (EC 3.1.1.1) represent a diverse group of hydrolases that catalyze the cleavage and formation of ester bonds. Although esterases are widely distributed among marine organisms, only microbial esterases are of paramount industrial importance. This article discusses the importance of marine microbial esterases, their biochemical and kinetic properties, and their stability under extreme conditions. Since culture-dependent techniques provide limited insights into microbial diversity of the marine ecosystem, therefore, genomics and metagenomics approaches have widely been adopted in search of novel esterases. Additionally, the article also explains industrial applications of marine bacterial esterases particularly for the synthesis of optically pure substances, the preparation of enantiomerically pure drugs, the degradation of human-made plastics and organophosphorus compounds, degradation of the lipophilic components of the ink, and production of short-chain flavor esters.

A critical review on marine serine protease and its inhibitors: A new wave of drugs?

Marine organisms are rich sources of enzymes and their inhibitors having enormous therapeutic potential. Among different proteolytic enzymes, serine proteases, which can be obtained from various marine organisms show a potential to biomedical application as thrombolytic agents. Although this type of proteases plays a crucial role in almost all biological processes, their uncontrolled activity often leads to several diseases. Accordingly, the actions of these types of proteases are regulated by serine protease inhibitors (SPIs). Marine SPIs control complement activation and various other physiological functions, such as inflammation, immune function, fibrinolysis, blood clotting, and cancer metastasis. This review highlights the potential use of serine proteases and their inhibitors as the new wave of promising drugs.

An overview on marine cellulolytic enzymes and their potential applications.

Marine-derived enzymes have recently gained attention particularly for industrial applications. Cellulose-degrading enzymes are among leading biocatalysts with potential utility in biorefineries. This review presents an account of the cellulase production by marine sources from microorganisms including bacteria, yeasts, and molds to marine invertebrates such as protist, rotifer, mollusks, arthropods, and echinoderms. Cellulose-degrading ability of marine invertebrates is attributed to the production of endogenous cellulases and activities by the symbionts. Specialized environments in marine including estuaries and mangroves are rich in lignocellulosic biomass and hence provide a feeding ground for cellulose digesters. Since cellulosic biomass is considered chemical and energy feedstock, therefore, cellulases with the ability to work under extreme environment are much needed to fulfill the demand of modern biotechnological industries. The review also discusses physicochemical parameters of marine-derived cellulases. Key Points: • Cellulolytic ability is widely distributed amongst marine organisms, yet very few have been studied for their biotechnological potential • Cellulase from marine organisms has been demonstrated as a successful agent in degradation of seaweed processing waste to low molecular fragments • Marine derived cellulases can find their application in green processes • Cellulases from marine sources exhibit high specific activity, thermostability, and other important biochemical properties and hence can contend well with the enzymes from terrestrial sources.

Marine microbial alkaline protease: An efficient and essential tool for various industrial applications.

With the modern world focusing on environmental friendly products, more and more chemical processes are being replaced by enzymatic methods. Alkaline proteases (APases) place more than 50% of the total world enzyme production. Marine microorganisms are capable of producing an extensive spectrum of APases which have important ecological roles and promising industrial applications. Marine microbial APases can meet the required market demand for various industrial processes due to their strong specificity, mild reaction conditions, environmental friendliness and easy inactivation or control in comparison with chemical catalysts. In this review, a bird's-eye view on recent research works in the field of APase production from marine microorganisms as well as their potential industrial applications. The effect of various physical and chemical parameters on marine microbial APase is discussed. Isolation, purification, optimum pH and temperature of marine microbial APases are also reported. We anticipate that this review will provide an outline of potential industrial application of marine microbial APases and open new avenues to help the academicians, researchers and industrialists.

Metabolites from Marine Microorganisms, Micro, and Macroalgae: Immense Scope for Pharmacology.

Marine organisms produce a large array of natural products with relevance in drug discovery. These compounds have biological activities such as antioxidant, antibacterial, antitumor, antivirus, anticoagulant, anti-inflammatory, antihypertensive, antidiabetic, and so forth. Consequently, several of the metabolites have made it to the advanced stages of clinical trials, and a few of them are commercially available. In this review, novel information on natural products isolated from marine microorganisms, microalgae, and macroalgae are presented. Given due research impetus, these marine metabolites might emerge as a new wave of promising drugs.

Marine bacterial chitinase as sources of energy, eco-friendly agent, and industrial biocatalyst.

Chitin is the richest renewable polymer carbohydrate in the marine environment and is an energetic source of nitrogen and carbon for marine organisms. Marine chitinolytic bacteria play a basic role in the nutrient cycling in the oceans by biodegradation of chitinous waste to useful form. Chitinase-producing bacteria from marine wastes increasing attention has received, and it serves two purposes: (i) reduce environmental hazards by waste management and (ii) increases generation of industrially important value-added products. This review aims to present the environmental pitfalls caused by marine waste and successes of chitinase-producing bacteria in employ waste for the generation of functional and valuable products, as well as to present importance role of chitinase-producing bacteria in industry. The focus is on isolation, purification, biochemical and enzymatic characteristics of chitinolytic bacteria in the oceans.

Future direction in marine bacterial agarases for industrial applications.

The marine ecosystem has been known to be a rich source of novel enzymes. Agarase is a key enzyme that can hydrolyze agar in the marine environment. Marine bacterial agarase has been isolated from various sources, such as sediments, coastal water, and deep sea and from the surface of crustaceans and seaweeds. This review presents an account of the agarase production of marine bacteria. General information about agar, agarase, isolation, and purification of marine bacterial agarases; the biochemical properties of native agarase from marine bacteria; the biochemical properties of recombinant marine bacterial agarases from engineered microorganisms; and the industrial future of marine bacterial agarases is analyzed. With recent biotechnological processes, researchers need novel functional enzymes like agarase from marine resources, such as marine bacteria, that can be used for diverse applications in the biotechnological industry. Marine bacterial agarases might be of significant interest to the industry because they are safe and are a natural source. This review highlights the potential of marine bacteria as important sources of agarase for application in various industries.

Thermostable marine microbial proteases for industrial applications: scopes and risks.

Thermostable proteases are important in biotechnological and industrial sectors, due to their stability against denaturing agents and chemicals. The feature that gives them such unique applicability is their reaction at high temperatures, which affords a high concentration of substrate, and less risk of microbial contamination. Nearly 65% of industrial proteases are isolated from marine microbial source, and they can significantly resist a wide range of organic solvents at high temperatures. The most important trait of marine organisms is their adaptability, which allows them to grow optimally in harsh environments such as high salt, temperatures, and pressure-the characteristics of deep-sea hot springs and geothermal sediments. However, proteases are immunogenic, and they can trigger inflammatory conditions in human; so their safety assessment prior to industrial usage is of paramount importance. This review focusses on marine-origin thermophilic proteases, their thermal resistance, scopes of their industrial applications, and risks.

Proximate composition and mineral contents in the body wall of two species of sea cucumber from Oman Sea.

The proximate composition and mineral contents of Stichopus horrens and Holothuria arenicola from Chabahar Bay were analyzed and investigated. During the present study, we aimed to demonstrate the nutritive value. The approximate percent composition of moisture, protein, fat, and ash were 92.8, 3.47, 0.4, and 3.33% in S. horrens and 93, 4.4, 0.6, and 2% in H. arenicola, respectively. Atomic absorption spectrophotometry of the ashes indicated the body wall of two species of sea cucumbers contained higher amounts of both macro minerals (92.5 mg/100 g Mg in S. horrens and 115 mg/100 g Mg in H. arenicola; 106.25 mg/100 g Ca in S. horrens and 83.25 mg/100 g Ca in H. arenicola) and trace elements (521.781 mg/100 g Fe in S. horrens; 60.354 mg/100 g Fe in H. arenicola, and 0.096 mg/100 g Zn in S. horrens; 0.04 mg/100 g Zn in H. arenicola). For both species, there were high content of protein and essential mineral. Also, they have low content of fat in the body wall of two species in the experiment.